Groundwater protection in mixed land-use aquifers

Compared with groundwater pollution episodes of a point-source nature, nonpoint-source contamination makes for particularly difficult policy design. This is especially true in the context of a mixed land-use aquifer, where the same pollutant may derive from various human land-use activities and in different concentrations. To data, the emprirical literature attempting to estimate the relative loadings of pollutants from alternative land uses is rather sparse. Yet this information is vital to a variety of numerical computer models used to predict likelihood of groundwater contamination, and the statistical results are useful in their own right for regional policy formation. Regression analysis is applied to estimate loadings of nitrate and sodium from various land uses. The model is then used to illustrate how well-intended local groundwater protection policies that fail to recognize land-use substitution and cross-pollutant effects may be misdirected.     

Preimplantation genetic diagnosis for single gene disorders: experience with five single gene disorders

We report our experience of 14 preimplantation genetic diagnosis (PGD) cycles in eight couples carrying five different single gene disorders, during the last 18 months. Diagnoses were performed for myotonic dystrophy (DM), cystic fibrosis (CF) [ΔF508 and exon 4 (621+1 G>T)], fragile X and CF simultaneously, and two disorders for which PGD had not been previously attempted, namely neurofibromatosis type 2 (NF2) and Crouzon syndrome. Diagnoses for single gene disorders were carried out on ideally two blastomeres biopsied from Day 3 embryos. A highly polymorphic marker was included in each diagnosis to control against contamination. For the dominant disorders, where possible, linked polymorphisms provided an additional means of determining the genotype of the embryo hence reducing the risk of misdiagnosis due to allele dropout (ADO). Multiplex fluorescent polymerase chain reaction (F-PCR) was used in all cases, followed by fragment analysis and/or single-stranded conformation polymorphism (SSCP) for genotyping. Embryo transfer was performed in 13 cycles resulting in one biochemical pregnancy for CF, three normal deliveries (a twin and a singleton) and one early miscarriage for DM and a singleton for Crouzon syndrome. In each case the untransferred embryos were used to confirm the diagnoses performed on the biopsied cells. The results were concordant in all cases. The inclusion of a polymorphic marker allowed the detection of extraneous DNA contamination in two cells from one case. Knowing the genotype of the contaminating DNA allowed its origin to be traced. All five pregnancies were obtained from embryos in which two blastomeres were biopsied for the diagnosis. Our data demonstrate the successful strategy of using multiplex PCR to simultaneously amplify the mutation site and a polymorphic locus, fluorescent PCR technology to achieve greater sensitivity, and two-cell biopsy to increase the efficiency and success of diagnoses. Copyright © 2002 John Wiley & Sons, Ltd.     

Towards a science of sustainable upland management in developing countries

Farmers attempting to subsist in tropical uplands often rely upon unsustainable resource use practices that lead to soil erosion, declining crop yields, and a loss of soil productivity capacity. Other uses of tropical uplands, including logging and conversion of forest to rangelands, have similar results. The undesirable effects of these actions are felt on-site, at the watershed level, and even nationally. Ecological cause-effect relationships are poorly understood, and few examples exist of the successful integration of ecological knowledge with upland development. It is hypothesized that recent results of ecological research could be applied to uplands management so that stable sustainable systems of human use may be established. A second hypothesis is that statistically reliable data can be obtained from experiments in upland situations, although natural variations of soils, weather, and vegetation are great. To test these hypotheses, research involving multinational collaboration among American and Southeast Asian scientists has begun. The objective of the work is to provide credible quantitative information to help policy and decision makers and resident farmers to plan and implement improved practices based on ecological principles.Some findings to date include:

The effect of biofuel production on swine farm methane and ammonia emissions

Methane (CH) and ammonia (NH3) are emitted to the atmosphere during anaerobic processing of organic matter, and both gases have detrimental environmental effects. Methane conversion to biofuel production has been suggested to reduce CH4 emissions from animal manure processing systems. The purpose of this research is to evaluate the change in CH4 and NH3 emissions in an animal feeding operation due to biofuel production from the animal manure. Gas emissions were measured from swine farms differing only in their manure-management treatment systems (conventional vs. biofuel). By removing organic matter (i.e., carbon) from the biofuel farms' manure-processing lagoons, average annual CH4 emissions were decreased by 47% compared with the conventional farm. This represents a net 44% decrease in global warming potential (CO2 equivalent) by gases emitted from the biofuel farms compared with conventional farms. However, because of the reduction of methanogenesis and its reduced effect on the chemical conversion of ammonium (NH4+) to dinitrogen (N2) gas, NH3 emissions in the biofuel farms increased by 46% over the conventional farms. These studies show that what is considered an environmentally friendly technology had mixed results and that all components of a system should be studied when making changes to existing systems.     

Variation in size of living articulated brachiopods with latitude and depth

Geographical variations in animal characters are one of the main subjects for study in macroecology. Variation with latitude has received special interest. Articulated brachiopods are possibly the commonest macrofossil with large variations in size of taxa through the fossil record. Here, we investigate trends in size of the 3 main orders of articulated brachiopod with latitude and depth. Data were insufficient to identify patterns in Thecideida (a micromorph taxon only recorded from low latitudes). Rhynchonellida had no clear trends in size with latitude or depth. Terebratulida exhibited hemispheric differences in size relations, with increasing length of species towards the pole in the south and no significant trend in the north. Tropical species were small (<20 mm length between 10°N and 10°S), and the largest species were found between 30° and 60° latitude in both hemispheres. There were no articulated brachiopods recorded from the high arctic, and support for a continuous trend in size with latitude was small or absent. In Terebratulida, there was a significant decrease in species length with depth of 1.7 mm per 100 m depth increase. These trends could be explained by competition for space and reduced availability of habitat with progressive depth beyond the continental shelf.     

Management of the UK HSE failure rate and event data

The UK Health and Safety Executive (HSE) requires failure rate data for the assessment of COMAH safety reports and in the implementation of its statutory functions relating to land use planning in the vicinity of major hazard sites. Many of the existing failure rates used by HSE were derived over 20 years ago, but have been subject to periodic review to ensure that they remain appropriate for modern planning enquiries or quantified risk assessments. HSE needs to be assured that its sources of data and their application continue to be fit to support its statutory duties.HSE has implemented a programme of work to be carried out by the Health and Safety Laboratory (HSL). This includes the development and maintenance of a single source of quality assured failure rate data, ideally accessible from the Internet, bringing together and updating existing failure rate data sources and reviewing new sources not previously available to HSE.A review of HSE’s current failure rate values is being carried out and this will be used to generate a single source of publically available failure rate data for use by both HSE and the public. This would help industry and HSE move toward a common position or understanding on failure rates. It would also help ensure that HSE professional advice is defensible and transparent.This paper will detail the progress made to date on the programme of work. It will also discuss the issues that arise as a consequence of changing failure rates and how HSE deals with these issues.     

Portable XRF analysis of occupational air filter samples from different workplaces using different samplers: final results, summary and conclusions

This paper concludes a five-year program on research into the use of a portable X-ray fluorescence (XRF) analyzer for analyzing lead in air sampling filters from different industrial environments, including mining, manufacturing and recycling. The results from four of these environments have already been reported. The results from two additional metal processes are presented here. At both of these sites, lead was a minor component of the total airborne metals and interferences from other elements were minimal. Nevertheless, only results from the three sites where lead was the most abundant metal were used in the overall calculation of method accuracy. The XRF analyzer was used to interrogate the filters, which were then subjected to acid digestion and analysis by inductively-coupled plasma optical-emission spectroscopy (ICP-OES). The filter samples were collected using different filter-holders or "samplers" where the size (diameter), depth and homogeneity of aerosol deposit varied from sampler to sampler. The aerosol collection efficiencies of the samplers were expected to differ, especially for larger particles. The distribution of particles once having entered the sampler was also expected to differ between samplers. Samplers were paired to allow the between-sampler variability to be addressed, and, in some cases, internal sampler wall deposits were evaluated and compared to the filter catch. It was found, rather surprisingly, that analysis of the filter deposits (by ICP-OES) of all the samplers gave equivalent results. It was also found that deposits on some of the sampler walls, which in some protocols are considered part of the sample, could be significant in comparison to the filter deposit. If it is concluded that wall-deposits should be analyzed, then XRF analysis of the filter can only give a minimum estimate of the concentration. Techniques for the statistical analysis of field data were also developed as part of this program and have been reported elsewhere. The results, based on data from the three workplaces where lead was the major element present in the samples, are summarized here. A limit of detection and a limit of quantitation are provided. Analysis of some samples using a second analyzer with a different X-ray source technology indicated reasonable agreement for some metals (but this was not evaluated for lead). Provided it is only necessary to analyze the filters, most personal samplers will provide acceptable results when used with portable XRF analysis for lead around applicable limit values.     

No Net Loss of Fish Habitat: A Review and Analysis of Habitat Compensation in Canada

The achievement of No Net Loss (NNL) through habitat compensation has rarely been assessed in Canada. Files relating to 124 Fisheries Act Section 35(2) authorizations issued by Fisheries and Oceans Canada for the harmful alteration, disruption, and destruction of fish habitat (HADD) were collected and reviewed. Data extracted from these files were pooled and analyzed to provide an indication of the types of HADDs that have been authorized in Canada, what habitats have been affected, and what habitat management approaches have been used when compensating for HADDs and monitoring and ensuring the success of the compensation. Determinations regarding the effectiveness of habitat compensation in achieving NNL were made. Impacts to 419,562 m² of fish habitat from the 124 authorized HADDs were offset by 1,020,388 m² of compensatory habitat. Eighty percent of the authorizations had compensation ratios (compensation area:HADD area) of 2:1 or less, and 25% of the authorizations had a compensation ratio that was less than 1:1. In-channel and riparian habitat were the most frequently impacted habitats. Urban development and roads and highways resulted in the greatest areal loss of habitat. The compensation option that was most often selected was the creation of in-kind habitat. The mean duration of post-construction monitoring programs was 3.7 years. Determinations of NNL could only be made for 17 authorizations as a result of poor proponent compliance with monitoring requirements and the qualitative assessment procedures used by the monitoring programs. Adequate resources, proper training, and standardized approaches to data management and monitoring programs are required to ensure that the conservation goal of NNL can be achieved in Canada.     

Organochlorine pesticide contamination in grassland-nesting passerines that breed in North America

Organochlorine pesticides and metabolites were measured in grassland-nesting passerines that breed in North America. We also examined testes of male birds for abnormalities that may have resulted from pesticide exposure. Forty-four of 99 individuals contained one or more organochlorine pesticides above the detection limit, representing nine of 10 species. The most prevalent compound detected was p,p'-DDE (minimum-maximum levels: 7.55-285.85 ng/g, carcass concentration). Insectivorous birds had significantly higher levels of p,p'-DDE than both omnivores and granivores. Birds that frequented moist grassland habitats had significantly higher levels of p,p'-DDE than those that frequented drier grassland habitats. No evidence of feminization was observed in any of the testes analyzed, however, other endpoint effects of contamination (e.g. hormone levels and immunological parameters) should be investigated in future studies.     

Nitrous oxide and ammonia fluxes in a soybean field irrigated with swine effluent

In the United States, swine (Sus scrofa) operations produce more than 14 Tg of manure each year. About 30% of this manure is stored in anaerobic lagoons before application to land. While land application of manure supplies nutrients for crop production, it may lead to gaseous emissions of ammonia (NH3) and nitrous oxide (N2O). Our objectives were to quantify gaseous fluxes of NH3 and N2O from effluent applications under field conditions. Three applications of swine effluent were applied to soybean [Glycine max (L.) Merr. 'Brim'] and gaseous fluxes were determined from gas concentration profiles and the flux-gradient gas transport technique. About 12% of ammonium (NH4-N) in the effluent was lost through drift or secondary volatilization of NH3 during irrigation. An additional 23% was volatilized within 48 h of application. Under conditions of low windspeed and with the wind blowing from the lagoon to the field, atmospheric concentrations of NH3 increased and the crop absorbed NH3 at the rate of 1.2 kg NH3 ha(-1) d(-1), which was 22 to 33% of the NH3 emitted from the lagoon during these periods. Nitrous oxide emissions were low before effluent applications (0.016 g N2O-N ha(-1) d(-1)) and increased to 25 to 38 g N2O-N ha(-1) d(-1) after irrigation. Total N2O emissions during the measurement period were 4.1 kg N2O-N ha(-1), which was about 1.5% of total N applied. The large losses of NH3 and N2O illustrate the difficulty of basing effluent irrigation schedules on N concentrations and that NH3 emissions can significantly contribute to N enrichment of the environment.